Guava Leaf Extract and Polyvinylpyrrolidone Hydrogel for Rabbit Oral Wounds

Authors

  • I Gusti Ayu Agung Bella Jayaningrum Prof. I.G.N.G. Ngoerah General Hospital
  • Agus Roy Rusly Hariantana Hamid Prof. I.G.N.G. Ngoerah General Hospital
  • I Gusti Putu Hendra Sanjaya Prof. I.G.N.G. Ngoerah General Hospital
  • Gede Wara Samsarga Prof. I.G.N.G. Ngoerah General Hospital
  • I Wayan Niryana Prof. I.G.N.G. Ngoerah General Hospital
  • I Made Darmajaya Prof. I.G.N.G. Ngoerah General Hospital

DOI:

https://doi.org/10.58344/locus.v5i2.5040

Keywords:

Wound, guava leaf ethanol extract, oral mucosa, fibroblasts, VEGF levels, IL-16 levels

Abstract

Wounds, caused by trauma, surgery, or accidents, can disrupt tissue integrity. A common complication in maxillofacial surgery is wound dehiscence. The combination of guava leaf (Psidium guajava Linn.) ethanol extract and polyvinylpyrrolidone (PVP) hydrogel is expected to accelerate oral mucosal wound healing by enhancing fibroblast proliferation, epithelialization, and VEGF levels, while reducing IL-6 levels. This research aims to evaluate the effectiveness of a combination gel of guava leaf ethanol extract and PVP hydrogel in accelerating oral mucosal wound healing. This experimental study used 28 rabbits divided into four groups: Group 1 (sutured wound + combination gel), Group 2 (unsutured wound + combination gel), Group 3 (sutured wound + placebo), and Group 4 (unsutured wound + placebo). A standardized incisional wound was created on the gingiva, and the gel was applied twice daily. Histopathological analysis was performed on days 3 and 14 to assess fibroblast count, epithelialization, VEGF, and IL-6 levels. The treatment group showed significantly higher fibroblast counts (64.50 ± 4.43 vs. 55.67 ± 4.04; p = 0.041), enhanced epithelialization, increased VEGF levels, and a 25% reduction in IL-6 levels on day 3 compared to controls. These findings support the gel's role in accelerating wound healing through angiogenesis and inflammation control. The combination of guava leaf ethanol extract and PVP hydrogel is a promising innovative therapy for accelerating wound healing, particularly in chronic or difficult-to-heal wounds, with minimal risk of excessive inflammation and scar tissue formation.

References

Abdulazeem, L., Tariq, A., & Abdalkareem Jasim, S. (2022). An investigation of vascular endothelial growth factor (VEGFR-1 and VEGFR-2) in burn wound healing. Archives of Razi Institute, 77(2), 747.

Buchsel, P. C. (2008). Polyvinylpyrrolidone-sodium hyaluronate gel (Gelclair). Expert Opinion on Drug Metabolism & Toxicology, 4(11), 1449–1454. https://doi.org/10.1517/17425255.4.11.1449

Budovsky, A., Yarmolinsky, L., & Ben-Shabat, S. (2015). Effect of medicinal plants on wound healing. Wound Repair and Regeneration, 23(2), 171–183. https://doi.org/10.1111/wrr.12274

Cao, X., Deng, Y., Xu, Z., Wang, T., Tang, B., & Han, J. (2025). A versatile natural gelatin-based hydrogel for emergency wound treatment. Biofabrication, 17(1), 15017. https://doi.org/10.1088/1758-5090/ad89ff

Carolino, M. V, Purnamasari, L., & Flores Dela Cruz, J. (2022). The antibacterial properties of Psidium guajava leaf extract as a wound healing agent. Biotropika, 10(2), 154–160. https://doi.org/10.21776/UB.BIOTROPIKA.2022.010.02.10

Cialdai, F., Risaliti, C., & Monici, M. (2022). Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Frontiers in Bioengineering and Biotechnology, 10, 958381. https://doi.org/10.3389/fbioe.2022.958381

Das, A., Abas, M., Biswas, N., Banerjee, P., Ghosh, N., & Rawat, A. (2019). A modified collagen dressing induces transition of inflammatory to reparative phenotype of wound macrophages. Scientific Reports, 9, 14688. https://doi.org/10.1038/s41598-019-51061-3

Delorino, S. B., Ogalesco, M. L., Rebadulla, K. R., Rongcales, M. T. A., Salubre, J. I. A., Talacay, M. K. S., & Tuballas, Z. B. (2021). Wound healing efficacy of guava leaf extract. Journal of Pharmaceutical Research International, 32(41), 27–35. https://doi.org/10.9734/jpri/2020/v32i4131041

Demidova-Rice, T. N., Hamblin, M. R., & Herman, I. M. (2012). Acute and impaired wound healing: Pathophysiology and current methods for drug delivery, Part 1: Normal and chronic wounds. Advances in Skin & Wound Care, 25(7), 304. https://doi.org/10.1097/01.ASW.0000416006.55218.D0

Ekom, S. E., & Tamokou, J.-D. D. (2018). Methanol leaves extract of Psidium guajava Linn. exhibited antibacterial and wound healing activities. International Journal of Current Microbiology and Applied Sciences, 7(7), 4008–4023. https://doi.org/10.20546/ijcmas.2018.707.467

Esmaeelinejad, M., & Esmaeelinejad, M. (2018). Maxillofacial fractures: From diagnosis to treatment. IntechOpen. https://doi.org/10.5772/INTECHOPEN.76166

Espinosa, M. C., Hohman, M. H., & Sivam, S. (2023). Oral and maxillofacial surgery, facial laceration repair. StatPearls.

Foster, D. S. (2021). Integrated spatial multiomics reveals fibroblast fate during tissue repair. Proceedings of the National Academy of Sciences, 118(41), e2110025118. https://doi.org/10.1073/pnas.2110025118

Gonzalez, A. C. D. O. (2016). Wound healing: A literature review. Anais Brasileiros de Dermatologia, 91(5), 614. https://doi.org/10.1590/ABD1806-4841.20164741

Hakim, R. F., Fakhrurrazi, F., & Zamzami, F. S. (2023). Comparison of the effect of guava (Psidium guajava L.) leaf extract with polyvinylpyrrolidone-sodium hyaluronate on the number of fibroblasts. Odonto: Dental Journal, 10(1), 61. https://doi.org/10.30659/odj.10.1.61-68

Hao, M., Ding, C., Sun, S., Peng, X., & Liu, W. (2022). Chitosan/sodium alginate/velvet antler blood peptides hydrogel promotes diabetic wound healing. Journal of Inflammation Research, 15, 4921–4938. https://doi.org/10.2147/JIR.S376692

Irfan-Maqsood, M. (2016). Classification of wounds: Know before research and clinical practice. Cell Therapy and Regenerative Medicine Journal, 1(2), 79. https://doi.org/10.15562/CTRM.21

Khan, T. U. (2022). Etiology and pattern of maxillofacial trauma. PLoS ONE, 17(9). https://doi.org/10.1371/journal.pone.0275515

Kumar, J. N., & Ravi, P. (2021). Postoperative care of the maxillofacial surgery patient. Oral and Maxillofacial Surgery Clinics, 239. https://doi.org/10.1007/978-981-15-1346-6_12

Liang, J., Ling, J., Sun, D., Wu, G., Ouyang, X., Wang, N., & Yang, G. (2024). Dextran-based antibacterial hydrogel dressings for accelerating infected wound healing. Advanced Healthcare Materials. https://doi.org/10.1002/adhm.202400494

Liu, Y. (2022). Fibroblasts: Immunomodulatory factors in refractory diabetic wound healing. Frontiers in Immunology, 13, 918223. https://doi.org/10.3389/fimmu.2022.918223

Maigoda, A. R., Refdanita, S., & Abidin, Z. (2022). Collagen synthesis in wound healing accelerated by herbal gel formulation. International Journal of Biological Macromolecules, 194, 567–576. https://doi.org/10.1016/j.ijbiomac.2022.02.087

Mascharak, S. (2021). Preventing Engrailed1 activation in fibroblasts yields wound regeneration without scarring. Science.

Naseer, S., Hussain, S., Naeem, N., Pervaiz, M., & Rahman, M. (2018). The phytochemistry and medicinal value of Psidium guajava (guava). Clinical Phytoscience, 4, 32. https://doi.org/10.1186/s40816-018-0093-8

Oksa, M. (2023). Postoperative wound dehiscence in mandibular fractures. Acta Odontologica Scandinavica, 81(7), 555–561. https://doi.org/10.1080/00016357.2023.2211156

Roman, R. (2023). Fibroblasts – Warriors at the intersection of wound healing and immunomodulation. Journal of Tissue Repair and Regeneration. https://doi.org/10.1016/j.jtrr.2023.03.001

Singh, V. (2012). The maxillofacial injuries: A study. National Journal of Maxillofacial Surgery, 3(2), 166. https://doi.org/10.4103/0975-5950.111372

Stunová, A., & Vištejnová, L. (2018). Dermal fibroblasts: A heterogeneous population with regulatory functions in wound healing. Cytokine & Growth Factor Reviews.

Sutrisno, E., Susilawati, E., Herdiana, G. B., & Noviyanti, E. (2024). Gel formulations of guava leaves (Psidium guajava L) ethanol extract as a wound healing of burns in rabbits. International Journal of Research in Community Service, 5(2), 76–81.

Wang, X., Coradin, T., & Hélary, C. (2018). Modulating inflammation in a cutaneous chronic wound model by IL-10 released from collagen–silica nanocomposites. Biomaterials Science, 6(2), 398–406. https://doi.org/10.1039/c7bm01024a

Wusiman, P., Maimaitituerxun, B., Guli, S., Saimaiti, A., & Moming, A. (2020). Epidemiology and pattern of oral and maxillofacial trauma. Journal of Craniofacial Surgery, 31(5), e517–e520.

Yadav, A. (2021). Principles of internal fixation in maxillofacial surgery. Oral and Maxillofacial Surgery Clinics, 1039–1051. https://doi.org/10.1007/978-981-15-1346-6_51

Yang, L., Zhang, L., Hu, J., Wang, W., & Liu, X. (2021). Promote anti-inflammatory and angiogenesis using a hyaluronic acid-based hydrogel with miRNA-laden nanoparticles. International Journal of Biological Macromolecules, 166, 166–178. https://doi.org/10.1016/j.ijbiomac.2020.10.163

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Published

2026-02-23

Issue

Vol. 5 No. 2 (2026): JURNAL LOCUS: Penelitian dan Pengabdian

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Articles

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Copyright (c) 2026 I Gusti Ayu Agung Bella Jayaningrum, Agus Roy Rusly Hariantana Hamid, I Gusti Putu Hendra Sanjaya, Gede Wara Samsarga, I Wayan Niryana, I Made Darmajaya

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