Deteksi Residu Malathion pada Tanaman Tomat Menggunakan Sensor LSPR Berbasis Nanobipiramida Emas
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Penelitian ini bertujuan mengembangkan sensor plasmonik berbasis gold nanobipyramids (GNBP) untuk mendeteksi residu malathion pada tanaman tomat (Solanum lycopersicum L.). GNBP disintesis menggunakan metode seed-mediated growth dan dikarakterisasi menggunakan spektroskopi UV-Vis, X-Ray Diffraction (XRD), dan Field Emission Scanning Electron Microscope (FESEM). Hasil karakterisasi UV-Vis menunjukkan dua puncak localized surface plasmon resonance (LSPR), yaitu transverse surface plasmon resonance (t-SPR) pada sekitar 560 nm dan longitudinal surface plasmon resonance (l-SPR) pada sekitar 740 nm yang menandakan terbentuknya struktur anisotropik nanobipiramida emas. Analisis XRD mengonfirmasi struktur kristal face centered cubic (FCC) khas emas, sedangkan citra FESEM menunjukkan morfologi bipiramida dengan distribusi ukuran yang relatif homogen. Pengujian sensitivitas sensor menunjukkan adanya perubahan intensitas absorbansi setelah interaksi antara GNBP dan ekstrak tomat yang mengandung malathion, yang mengindikasikan perubahan respons plasmonik akibat perubahan indeks bias lokal di sekitar nanopartikel. Pita l-SPR menunjukkan sensitivitas yang lebih tinggi dibandingkan pita t-SPR terhadap keberadaan malathion. Selain itu, pengujian repetabilitas dan stabilitas menunjukkan bahwa sensor memiliki respons yang konsisten dan stabil selama pengukuran berulang hingga 600 detik. Hasil penelitian menunjukkan bahwa sensor plasmonik berbasis GNBP memiliki potensi yang baik untuk aplikasi deteksi residu pestisida pada bidang keamanan pangan dan pemantauan lingkungan.
Botnaru, A. A., et al. (2025). Innovative analytical approaches for food pesticide residue detection: Towards One Health-oriented risk monitoring. Journal of Xenobiotics, 15(5). https://doi.org/10.3390/jox15050151
Campu, A., et al. (2019). Multimodal biosensing on paper-based platform fabricated by plasmonic calligraphy using gold nanobipyramids ink. Frontiers in Chemistry, 7, Article 55. https://doi.org/10.3389/fchem.2019.00055
Gai, T., Nie, J., Ding, Z., Wu, W., & Liu, X. (2023). Progress of rapid detection of pesticides in fruits and vegetables. Frontiers in Food Science and Technology, 3, Article 1253227. https://doi.org/10.3389/frfst.2023.1253227
Ghosh, S., et al. (2022). The application of rapid test paper technology for pesticide detection in horticulture crops: A comprehensive review. Chemical and Biological Technologies in Agriculture, 9, Article 48. https://doi.org/10.1186/s43088-022-00248-6
Guo, R., et al. (2023). Multicolor visual detection of deoxynivalenol in grain based on magnetic immunoassay and enzymatic etching of plasmonic gold nanobipyramids. Toxins, 15(6), Article 351. https://doi.org/10.3390/toxins15060351
He, Y., Xiao, S., Dong, T., & Nie, P. (2019). Gold nanoparticles with different particle sizes for the quantitative determination of chlorpyrifos residues in soil by SERS. International Journal of Molecular Sciences, 20(11), Article 2817. https://doi.org/10.3390/ijms20112817
Iwantono, I., et al. (2025). Detection of malathion in Ipomoea aquatica using a plasmonic sensor based on Ag-modified gold nanobipyramids. Journal of Applied Agricultural Science and Technology, 9(1), 85–98. https://doi.org/10.55043/jaast.v9i1.369
Liu, W., Liu, D., Zhu, Z., Han, B., Gao, Y., & Tang, Z. (2014). DNA induced intense plasmonic circular dichroism of highly purified gold nanobipyramids. Nanoscale, 6(9), 4498–4502. https://doi.org/10.1039/C4NR00166D
Liu, X., et al. (2024). Non-linear responses via agglomeration and aggregation of gold nanoparticles for surface-enhanced Raman spectroscopy (SERS) coupled with chemometric analysis for chlorpyrifos detection. Food Chemistry, 455, Article 139944. https://doi.org/10.1016/j.foodchem.2024.139944
Mikac, L., Rigó, I., Škrabi?, M., Ivanda, M., & Veres, M. (2022). Comparison of glyphosate detection by surface-enhanced Raman spectroscopy using gold and silver nanoparticles at different laser excitations. Molecules, 27(18), Article 5767. https://doi.org/10.3390/molecules27185767
Mnif, W., Hassine, A. I. H., Bouaziz, A., Bartegi, A., Thomas, O., & Roig, B. (2011). Effect of endocrine disruptor pesticides: A review. International Journal of Environmental Research and Public Health, 8(6), 2265–2303. https://doi.org/10.3390/ijerph8062265
Moslemi, A., Sansone, L., Esposito, F., Campopiano, S., Giordano, M., & Iadicicco, A. (2024). Optical fiber probe based on LSPR for the detection of pesticide thiram. Optics & Laser Technology, 175, Article 110882. https://doi.org/10.1016/j.optlastec.2024.110882
Nafisah, S., et al. (2021). Effect of additive acid on seeded growth of gold nanobipyramids. Journal of Physics and Chemistry of Solids, 148, Article 109764. https://doi.org/10.1016/j.jpcs.2020.109764
Putra, R. A., Fadlly, T. A., Yakob, M., Jalil, Z., & Rahwanto, A. (2021). Biosensor berbasis surface plasmon resonance (SPR). Deepublish.
Syafrudin, M., et al. (2021). Pesticides in drinking water: A review. International Journal of Environmental Research and Public Health, 18(2), Article 468. https://doi.org/10.3390/ijerph18020468
Tsagkaris, A. S., Pulkrabova, J., & Hajslova, J. (2021). Optical screening methods for pesticide residue detection in food matrices: Advances and emerging analytical trends. Foods, 10(1), Article 88. https://doi.org/10.3390/foods10010088
Yoo, S., Nam, D. H., Singh, T. I., Leem, G., & Lee, S. (2022). Effect of reducing agents on the synthesis of anisotropic gold nanoparticles. Nano Convergence, 9(1), Article 1. https://doi.org/10.1186/s40580-021-00296-1
Yu, H., et al. (2021). Nylon membranes modified by gold nanoparticles as surface-enhanced Raman spectroscopy substrates for several pesticides detection. RSC Advances, 11(39), 24183–24189. https://doi.org/10.1039/D1RA03490A
Zainul, R. (2025). Elektrokimia dalam teknologi sensor dan deteksi analit. PT RajaGrafindo Persada.
Zhou, W., Li, M., & Achal, V. (2025). A comprehensive review on environmental and human health impacts of chemical pesticide usage. Environmental Challenges. https://doi.org/10.1016/j.emcon.2024.100410
